WebMar 16, 2024 · Serum EBV VCA IgM antibody was detected using an enzyme-linked immunosorbent assay (ELISA) in vitro diagnostic kit (Thunderbolt, Gold Standard Diagnostics, CA) for the quantitative measurement of IgM antibodies against the VCA antigens p23 and p18 of EBV. Serum EBV VCA IgM positivity was defined as serum … WebSerum samples were collected upon diagnosis, after 1-3 and 4-6 months and at the end of treatment, or at disease recurrence. RT-PCR determination of cf-DNA and EBV DNA were performed using amplification of BAMH1W region of EBV genome and of human β-globin gene. Results were analyzed for correlation with clinical and pathological characteristics.
Serum Epstein-Barr virus DNA, detected by droplet …
WebPost-Transplant, Epstein-Barr Virus DNA, Quantitative Real-Time PCR, Serum - Monitoring EBV DNA levels by quantitative PCR in patients at risk of EBV-associated … WebEBVPCR Methodology Qualitative Polymerase Chain Reaction Performed Sun-Sat Reported 1-4 days New York DOH Approval Status This test is New York DOH approved. Specimen Required Patient Preparation Collect Lavender (K 2 EDTA), Pink (K 2 EDTA), or Serum Separator Tube (SST). miller syncrowave 300 torch
Cytomegalovirus DNA, Qualitative, Real-Time PCR
WebJul 30, 2024 · With a PCR pre-amplification of the target (45 min), the assay achieved a sensitivity of 7.1 × 10 −14 M (∼42 000 copies per μl) both in plasmid and plasmid-spiked samples. The assay attained a high specificity in the presence of various bacteria and applicability in EBV Burkitt's lymphoma serum samples. WebRecently, EBV PCR has been added as a diagnostic tool. Positive EBV PCR has been demonstrated in the serum of patients with primary EBV infections and EBV reactivation. … WebClinical Significance. Monitoring EBV DNA levels by quantitative PCR in patients at risk of EBV-associated lymphoproliferative disorders may allow timely recognition of virus reactivation and permit installment of antiviral treatment. This is a quantitative molecular test, with a linear range of 200-2,000,000 copies/mL. miller syncrowave 210 lowest amperage