Web2 days ago · Subsequently, samples were incubated with the primary antibodies of mouse anti-α-actinin (1:200) and rabbit anti-CX-43 (1:200), and rabbit anti-cTnT (1:200) respectively at 4 °C overnight. WebThe dots were labeled positive with the α actinin antibody, and their sizes were similar to those of the I-bands in the cTnT +/+ phalloidin staining; thus we conclude that these dots correspond ...

(PDF) Document S1. Figures S1–S4

WebDownload scientific diagram (a) iPSC-CMs stained positive for cardiac troponin T (cTnT, red), α-actinin (green), and DAPI (blue). Scale bar, 25 μm. (b) Statistical comparison of nuclear area ... how much ppa should i get

Implementing High Sensitivity Cardiac Troponin Assays

WebThis model consisted of iPS-derived human atrial cardiomyocytes plated onto a micropatterned linear 1D spiral design of Matrigel. The CV (34-41 cm/s) of the in vitro model was nearly five times ... WebAug 6, 2010 · Fibroblasts transduced with GMT expressed GFP, cTnT, and actinin after 1 week at the same level as fibroblasts isolated from explant cultures (Figures 3 B and 3C). Similar results were obtained on introduction of GMT into adult cardiac fibroblasts, with full formation of sarcomeric structures ( Figure 3 D; Figure S2 ). WebMay 29, 2012 · The cardiomyocytes exhibited normal cardiac sarcomere organization, demonstrated by immunofluorescent staining of α-actinin, MLC2a, and cTnT (Fig. 4A and Fig. S4 D–G). Scanning electron microscopy also identified cells with myofibrillar bundles and transverse Z-bands (Fig. 4B and Fig. S4H) and cells enriched in mitochondria (Fig. 4B). how much ppa does a full time teacher get